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Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
 

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Article DOI: 10.3791/57390-v • 10:14 min • May 4th, 2018
May 4th, 2018 •
1, 2, 1, 1
1Invasive Insect Biocontrol and Behavior Laboratory, Agricultural Research Service, United States Department of Agriculture, 2Horticultural Research Laboratory, Agricultural Research Service, United States Department of Agriculture

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This article demonstrates novel techniques developed for oral delivery of double-stranded RNA (dsRNA) through the vascular tissues of plants for RNA interference (RNAi) in phloem sap feeding insects.

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Double-stranded RNA Oral Delivery RNA Interference Phloem Plant-sap-feeding Hemipteran Insects Gene-specific DsRNAs Nucleic Acid Mediated Gene Silencing Brown Marmorated Stink Bug Harlequin Bug Asian Citrus Psyllid Protocol BMSB Nymphs Certified Organic Green Beans Sodium Hypochlorite Solution Double-distilled H2O Washes Micro-centrifuge Tube
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