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CRISPR 指南对小鼠前列腺病毒传递的基因改变

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Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration

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CRISPR 指南对小鼠前列腺病毒传递的基因改变

DOI:

10.3791/57525-v

•

06:21 min

•

April 27, 2018

•

Maria Riedel, Martin F. Berthelsen, Latifa Bakiri, Erwin F. Wagner, Martin K. Thomsen

¹Department of Clinical Medicine,Aarhus University, ²GDD, Cancer Cell Biology Program,National Cancer Research Center (CNIO)

Capítulos

00:04Título
00:47Surgical Preparations
01:40Virus Delivery to the Murine Prostate
04:35Results: Loss of Pten Expression in the Prostate Using CRISPR/Cas9 Gene Editing
05:45Conclusion

Summary

Tadução automática

该协议描述了一种新建立的方法, 病毒传递到小鼠前列腺。使用 CRISPR/Cas9 技术, 基因过度表达, 或 recombinase 分娩, 该技术允许基因表达的原位改变, 并实施了新的小鼠模型前列腺癌。

Tags

Keywords: CRISPR Gene Alteration Murine Prostate Virus Delivery Prostate Cancer In Vivo Gene Editing Surgical Procedure Anesthesia Aseptic Technique Abdominal Incision Seminal Vesicle Insulin Syringe Gene Expression

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