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JoVE Journal Neuroscience
Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila
 

Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila

Article DOI: 10.3791/57701-v • 06:49 min • June 2nd, 2018
June 2nd, 2018 •
1, 2
1Division of Biological Science, Graduate School of Science, Nagoya University, 2Department of Molecular Genetics, Institute of Life Science, Kurume University

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This paper describes a protocol for ex vivo calcium imaging of the Drosophila brain. In this method, natural or synthetic compounds can be applied to the buffer to test their ability to activate particular neurons in the brain.

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Keywords: Ex Vivo Calcium Imaging Drosophila Endocrine Signaling Brain Responses Neuroscience Tissue Dissection Imaging Chamber GCaMP Fluorescence Imaging
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