Journal
/
Medicina
/
Genetic Analysis of Hereditary Transthyretin Ala97Ser Related Amyloidosis
JoVE Journal
Medicina
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Medicina
Genetic Analysis of Hereditary Transthyretin Ala97Ser Related Amyloidosis

Genetic Analysis of Hereditary Transthyretin Ala97Ser Related Amyloidosis

DOI:
10.3791/57743-v

•

06:33 min

•

June 09, 2018

•

, , , ,
1Department of Traditional Chinese Medicine, Division of Chinese Acupuncture and Traumatology,Chang Gung Memorial Hospital Linkou Medical Center and Chang Gung University College of Medicine, 2Department of Neurology,Chang Gung Memorial Hospital Linkou Medical Center and Chang Gung University College of Medicine

Capítulos

  • 00:04Título
  • 00:42DNA Extraction from Peripheral Blood
  • 03:28Genetic Analyses of Mutations
  • 05:38Results: Missense Mutation Visible via Sequence Chromatogram
  • 06:11Conclusion

Summary

Tadução automática

Tadução automática

Here, we present a protocol to confirm the presence of point mutation for the diagnosis of hereditary transthyretin amyloidosis, using Ala97Ser, the most common endemic mutation in Taiwan, as an example.

Tags

Genetic AnalysisHereditary TransthyretinAla97SerAmyloidosisDNA ExtractionPCRTransthyretin AmyloidosisPrognosisClinical LaboratoryBlood SamplePronaseRNaseIsopropyl AlcoholEthanolDNA PelletDNA Polymerase

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

FISH for Pre-implantation Genetic Diagnosis

Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data

The Monoiodoacetate Model of Osteoarthritis Pain in the Mouse

Induction of Accelerated Atherosclerosis in Mice: The “Wire-Injury” Model

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Comparing Bibliometric Analysis Using PubMed, Scopus, and Web of Science Databases

Integrating Augmented Reality Tools in Breast Cancer Related Lymphedema Prognostication and Diagnosis

A Non-Invasive Method for Generating the Cyclic Loading-Induced Intra-Articular Cartilage Lesion Model of the Rat Knee

Employing the Forced Oscillation Technique for the Assessment of Respiratory Mechanics in Adults

A Murine Model of Hemodialysis Access-Related Hand Dysfunction

Read Article