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大鼠外周全血细胞免疫表型和因子生成的单细胞分析

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

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大鼠外周全血细胞免疫表型和因子生成的单细胞分析

DOI:

10.3791/57780-v

•

12:36 min

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June 26, 2018

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Ryan M. Baxter*¹, Daniel S. Kong*¹, Josselyn E. Garcia-Perez, William E. O’Gorman, Elena W.Y. Hsieh³

¹Department of Immunology and Microbiology,University of Colorado School of Medicine, ²OMNI Biomarkers, Development Sciences,Genentech, ³Department of Pediatrics, Division of Allergy and Immunology,University of Colorado School of Medicine

Capítulos

00:04Título
01:10Stimulation and Processing of Peripheral Whole Blood
05:27Barcode of Lysed/Fixed Blood Cells
09:44Results: Experimental Workflow and Representative Gating Strategy for the Identification of CD14hi Monocytes
11:50Conclusion

Summary

Tadução automática

在这里, 我们描述了一个单细胞蛋白质组学方法, 以评估免疫表型和功能 (细胞因子诱导) 改变周围全血标本, 分析通过大规模细胞术。

Tags

Keywords: Single-cell Analysis Immunophenotype Cytokine Production Peripheral Whole Blood Mass Cytometry Autoimmunity Cell Frequency Cytokine Production Systems Immunology Autoimmune Disease SLE Whole Blood Processing Ruxolitinib Lyse/fix Buffer Cell Recovery Antibody Staining

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