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操纵活细胞构建稳定的3D 蜂窝组件, 无需人工支架

JoVE Journal
Bioquímica

This content is Free Access.

JoVE Journal Bioquímica

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

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操纵活细胞构建稳定的3D 蜂窝组件, 无需人工支架

DOI:

10.3791/57815-v

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07:09 min

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October 26, 2018

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Takehiro Yamazaki*¹, Hiroaki Taniguchi*², Shoto Tsuji, Shiho Sato, Takahiro Kenmotsu, Kenichi Yoshikawa, Koichiro Sadakane

¹Faculty of Life and Medical Sciences,Doshisha University, ²The Institute of Genetics and Animal Breeding,Polish Academy of Sciences

Capítulos

00:04Título
00:48Preparation of Cells
02:41Preparation of Dextran
03:18Preparation for Laser and Microscopy
03:55Cell Manipulation Using the Laser Trapping System
05:30Construction of a 3-Dimensional Cell Structure
06:10Results: Analysis Stable 3D Cellular Assembly
06:35Conclusion

Summary

Tadução automática

我们展示了一种新的方法来构建一个基于单细胞的3维 (3D) 装配体, 而无需人工脚手架。

Tags

Keywords: 3D Cellular Assembly Cell Manipulation Hydrophilic Polymer Dextran NAMRU Mouse Mammary Gland Epithelial Cells Trypsin DMEM FBS Penicillin-Streptomycin Centrifugation Cryopreservation Laser Camera LED Focus Adjust Moving Stage

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