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The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan
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JoVE Journal Genética
The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

DOI:
10.3791/57819-v

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11:58 min

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June 29, 2018

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, , , ,
1Department of Biomedical Genetics,University of Rochester Medical Center, 2Department of Biostatistics and Computational Biology,University of Rochester Medical Center, 3Non-Clinical Statistics,Bristol-Myers Squibb, 4Department of Microbiology and Immunology,University of Rochester Medical Center

Capítulos

  • 00:04Título
  • 00:51Replica Set Method for Scoring C. elegans Longevity: Preparing the Plates
  • 03:33Replica Set Method for Scoring C. elegans Longevity
  • 06:33Traditional Method for Scoring C. elegans Longevity
  • 09:48Results: Sensitivity and Application of Replica Set Lifespan Analysis
  • 11:13Conclusion

Summary

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Here we describe the Replica Set method, an approach to quantitatively measure C. elegans lifespan/survival and healthspan in a high-throughput and robust manner, thus allowing screening of many conditions without sacrificing data quality. This protocol details the strategy and provides a software tool for analysis of Replica Set data.

Tags

Caenorhabditis ElegansLifespanAgingGenetic RelationshipsHigh-throughputReplica Set MethodRNAiSub-library96-well PlateBacteria CultureLB MediumAmpicillinTetracyclineAgar PlateDeep-welled PlateShaking IncubationControl Wells

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