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In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses
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JoVE Journal Bioengenharia
In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses

In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses

DOI:
10.3791/57823-v

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07:26 min

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December 05, 2019

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, , , ,
1Chair of Bioprocess Engineering, Department of Biotechnology,Technische Universität Berlin, 2SOPAT GmbH

Capítulos

  • 00:05Título
  • 01:09Overview of Probe
  • 01:40Off-line Measurement
  • 03:43Particle Identification
  • 05:11On-Line Measurement
  • 06:03Results: Cumulative Single-cell Size Distribution of S. Cerevisiae
  • 06:39Conclusion

Summary

Tadução automática

Tadução automática

A photo-optical in situ microscopy device was developed to monitor the size of single cells directly in the cell suspension. The real-time measurement is conducted by coupling the photo-optical sterilizable probe to an automated image analysis. Morphological changes appear with dependence on the growth state and cultivation conditions.

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Keywords: In Situ MicroscopyReal-timeSingle-cell MorphologyBioprocessesAutomated Image RecognitionCell ConcentrationFilamentous FungiYeastProbeCCD CameraMeasurement GapFocusCulture BrothPopulation HeterogeneityProcess YieldsProduct QualityContaminationCellular StructuresShapeCell Agglomeration

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