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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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JoVE Journal Genética
Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

DOI:
10.3791/57889-v

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11:22 min

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August 26, 2018

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, , , , ,
1Department of Genetics and Genome Sciences,Case Western Reserve University, 2Department of Endocrinology,Dongfang Hospital of Beijing University of Chinese Medicine, 3Case Comprehensive Cancer Center,Case Western Reserve University, 4Center for RNA Science and Therapeutics,Case Western Reserve University

Capítulos

  • 00:04Título
  • 01:32Sample Pretreatment
  • 05:00In Situ Hybridization Assay
  • 09:14Results: Use of RNA In Situ Hybridization Assay to Analyze Alternative Pre-mRNA Splicing in Mouse Brain Sections
  • 10:39Conclusion

Summary

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An in situ hybridization (ISH) protocol that uses short antisense oligonucleotides to detect alternative pre-mRNA splicing patterns in mouse brain sections is described.

Tags

Alternative Pre-mRNA SplicingRNA In Situ HybridizationMouse Brain SectionsQuantitative AnalysisIsoform-specific DetectionExon InclusionNF1 Exon 23aSlide PreparationHybridization Assay

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