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Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level
JoVE Journal
Biologia
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JoVE Journal Biologia
Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level
DOI:

08:56 min

July 17, 2018

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Capítulos

  • 00:04Título
  • 00:41Coverslip Cleaning and Functionalization
  • 03:22Flow Cell Assembly
  • 04:45Single Molecule Visualization
  • 07:20Results: Qdot705-labeled ORC Binding on λ-ARS317
  • 08:04Conclusion

Summary

Tadução automática

Here, we present a protocol to study DNA-protein interactions by total internal reflection fluorescence microscopy (TIRFM) using a site-specifically modified λ DNA substrate and a Quantum-dot labeled protein.

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