Journal
/
Genética
/
利用下一代序列染色体构象捕获 (4 c 序列) 的高通量识别基因调控数列
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
Please note that all translations are automatically generated. Click here for the English version.

利用下一代序列染色体构象捕获 (4 c 序列) 的高通量识别基因调控数列

DOI:
10.3791/58030-v

•

09:06 min

•

October 05, 2018

•

, ,
1Division of Dermatology, Center for Pharmacogenomics, Center for the Study of Itch, Department of Medicine,Washington University School of Medicine

Capítulos

  • 00:04Título
  • 00:45Formaldehyde Cross-linking and Cell Lysis
  • 01:59First Restriction Digestion and First Ligation
  • 05:07Cross-linking Reversion, Chromatin Isolation, and Further Analyses
  • 07:42Results: Representative Genomic Track of 4C-seq Read Coverage
  • 08:29Conclusion

Summary

Tadução automática

Tadução automática

基因和调控元素之间的物理相互作用的识别是具有挑战性的, 但已被染色体构象捕获方法所促进。这种对 4 c 序列协议的修改可以减少 pcr 模板的过度放大, 并通过加入限制酶消化步骤来最大限度地提高 mappability 的读数。

Tags

4C-seqChromatin InteractionsGene Regulatory SequencesNext-generation SequencingTranscriptional RegulationPromoter-enhancer InteractionsCross-linkingRestriction DigestionCell Lysis

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

适用于下一代测序的植物细菌DNA富集方法的优化与比较分析

核密度分析的天然染色质沉淀测序文库的生成

通过重叠2方法高通量识别协同药物组合

有针对性的下一代测序和生物信息学管道评价宪政疾病的遗传决定因素

用于下一代测序的近全长 HIV-1 Proviruses 的扩增

使用RNA测序识别循环RNA

使用染色质免疫沉淀和高通量测序（ChIP-seq）发现沙门氏菌生物膜中的CsgD监管目标

通过定量染色体形成捕获（4C）鉴定胚胎体增强剂-促进者接触

使用下一代测序来鉴定与CD4启动子附近CAS9诱导的双链断裂修复相关的突变

基于半导体的下一代测序平台上非整倍体的植入前基因检测

Read Article