Journal
/
Medicina
/
Targeted and Selective Treatment of Pluripotent Stem Cell-derived Teratomas Using External Beam Radiation in a Small-animal Model
JoVE Journal
Medicina
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Medicina
Targeted and Selective Treatment of Pluripotent Stem Cell-derived Teratomas Using External Beam Radiation in a Small-animal Model

Targeted and Selective Treatment of Pluripotent Stem Cell-derived Teratomas Using External Beam Radiation in a Small-animal Model

DOI:
10.3791/58115-v

•

05:08 min

•

February 17, 2019

•

, , , , , ,
1Stanford Cardiovascular Institute,Stanford University School of Medicine, 2Department of Medicine, Division of Cardiology,Stanford University School of Medicine, 3Medical Service, Cardiology Section,Veteran Affairs Palo Alto Health Care System, 4Department of Pathology,Stanford University School of Medicine, 5Department of Radiology, Molecular Imaging Program,Stanford University School of Medicine, 6Peking University Shenzhen Health Science Institute, 7Department of Radiation Oncology,Stanford University School of Medicine

Capítulos

  • 00:04Título
  • 00:57Pluripotent Stem Cell (PSC) Transplantation, Bioluminescence Imaging (BLI), and Teratoma Irradiation
  • 03:48Results: Representative Successful Tumor Induction and Treatment
  • 04:25Conclusion

Summary

Tadução automática

Tadução automática

Research on treatment strategies for pluripotent stem cell-derived teratomas is important for the clinical translation of stem cell therapy. Here, we describe a protocol to, first, generate stem cell-derived teratomas in mice and, then, to selectively target and treat these tumors in vivo using a small-animal irradiator.

Tags

Stem CellTeratomaExternal Beam RadiationSmall Animal ModelIPSCsBioluminescence ImagingMatrigelAthymic Nude MiceCell TherapyTargeted Treatment

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/58115?article_type=v

Protocol for Long Duration Whole Body Hyperthermia in Mice
check_url/pt/58115?article_type=v

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
check_url/pt/58115?article_type=v

Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model
check_url/pt/58115?article_type=v

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur
check_url/pt/58115?article_type=v

Radiation Planning Assistant – A Streamlined, Fully Automated Radiotherapy Treatment Planning System
check_url/pt/58115?article_type=v

A Small Animal Model of Ex Vivo Normothermic Liver Perfusion
check_url/pt/58115?article_type=v

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography
check_url/pt/58115?article_type=v

Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.
check_url/pt/58115?article_type=v

Ultrasound-Guided Induced Pluripotent Stem Cell-Derived Cardiomyocyte Implantation in Myocardial Infarcted Mice
check_url/pt/58115?article_type=v

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Read Article