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耦合星形胶质细胞网络的大小、形状和方向性分析
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Neurociência
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JoVE Journal Neurociência
Analyzing the Size, Shape, and Directionality of Networks of Coupled Astrocytes
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耦合星形胶质细胞网络的大小、形状和方向性分析

DOI:
10.3791/58116-v

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10:10 min

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October 04, 2018

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, ,
1Groupe de Recherche sur le Système Nerveux Central, and Département de Neurosciences,Université de Montréal, 2Faculté de Médecine Dentaire,Université de Montréal

Capítulos

  • 00:04Título
  • 00:30Data Preparation
  • 02:32Cell Counting
  • 04:10Measuring Astrocytic Network Area
  • 05:04Determining the Main Direction Vector
  • 06:52Placing the Analyzed Networks in the Nucleus of Interest
  • 09:11Results: Astrocytic Networks Labeled with Biocytin in NVsnpr
  • 09:49Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们提出了一个评估胶质网络组织的协议。所述方法最大限度地减少偏倚, 以提供这些网络的描述性度量, 如细胞计数、大小、面积和原子核内的位置。各向异性的评估与矢量分析。

Tags

Keywords: Astrocytic NetworksAstrocytesFIJIImageJZ StackMax Intensity ProjectionBackground SubtractionNoise ReductionBinary ImageCell CountingMorphology Analysis

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