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哺乳动物轴突再生: 成年小鼠背根神经节体内电穿孔的研究

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

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哺乳动物轴突再生: 成年小鼠背根神经节体内电穿孔的研究

DOI:

10.3791/58171-v

•

06:17 min

•

September 01, 2018

•

Qiao Li, Cheng Qian, Feng-Quan Zhou²

¹Department of Orthopaedic Surgery,Johns Hopkins University School of Medicine, ²The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine

Capítulos

00:04Título
00:38Surgical Exposure of the L4 and L5 DRGs
01:37DRG Injection and Electroporation
02:23Sciatic Nerve Crush
03:07DRG and Sciatic Nerve Harvest and Imaging
04:23Results: EGFP-expressing Axons in the Sciatic Nerve and Spinal Cord
05:35Conclusion

Summary

Tadução automática

电穿孔是一种有效的方法, 将兴趣基因传递给细胞。本文应用这种方法对成年小鼠背根神经节的神经元进行了研究, 并对其在体内的轴突再生模型进行了描述。

Tags

In Vivo Electroporation Adult Mouse Dorsal Root Ganglion Gene Transfection Axon Regeneration Sensory Neurons Sciatic Nerve Crush Nerve Injury Gene Overexpression Gene Knockdown

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