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高通量荧光素酶法评价单周转蛋白酶 PCSK9 的蛋白质水解
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Bioquímica
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JoVE Journal Bioquímica
A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9
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高通量荧光素酶法评价单周转蛋白酶 PCSK9 的蛋白质水解

DOI:
10.3791/58265-v

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08:14 min

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August 28, 2018

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, ,
1Division of Cardiology, Department of Medicine,Zuckerberg San Francisco General and University of California San Francisco, 2Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute,University of California San Francisco

Capítulos

  • 00:04Título
  • 00:43High-throughput Luciferase-based Proteolysis Assay
  • 02:50Transfection (Day 1)
  • 04:32Assay (Day 2)
  • 06:41Results: Alterations in PCSK9 Proteolytic Activity are Common in Clinical Populations
  • 07:52Conclusion

Summary

Tadução automática

Tadução automática

本协议提出了一种评估细胞内一种内在的低活性、单周转蛋白酶的蛋白水解活性的方法。具体来说, 该方法用于评价 PCSK9 的蛋白水解活性, 这是脂代谢的关键驱动力, 其蛋白水解活性是其最终胆固醇功能所必需的。

Tags

PCSK9ProteolysisLuciferase AssayHigh-throughputAtherosclerotic Heart DiseaseProteaseHEK293T CellsTransfectionCell CultureBiochemical System

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