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1Janssen R&D, 2Department of Microbiology and Immunology,University of North Carolina, 3UNC Food Allergy Initiative,University of North Carolina, 4Department of Pediatrics,University of North Carolina, 5Department of Chemistry,University of Alberta, 6Department of Molecular Medicine,Scripps Research Institute, 7Department of Medical Microbiology and Immunology,University of Alberta
Capítulos
- 00:04Título
- 01:13Protein Antigen-PEGylated Lipid Conjugation
- 02:57Liposome Extrusion
- 04:24Calcium Flux Monitoring of Antigenic Liposome-induced B Cell Activation
- 06:33Mouse Peanut Sensitization and Ara h 2 (Ah2) Antigenic Liposome Challenge
- 08:26Results: Representative Allergic Response Analyses
- 09:39Conclusion
Described is the preparation of antigenic liposomal nanoparticles and their use in stimulating B-cell activation in vitro and in vivo. Consistent and robust antibody responses led to the development of a new peanut allergy model. The protocol for generating antigenic liposomes can be extended to different antigens and immunization models.
