Journal
/
/
Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
DOI:

06:01 min

January 07, 2019

, , ,

Capítulos

  • 00:04Título
  • 00:28Extracellular Protein Microarray Printing
  • 01:55Preparation of Multivalent Bait Complexes
  • 03:09Extracellular Protein Microarray Screening
  • 04:36Results: Evaluation of Extracellular Protein Microarray Slide Printing and Screen of an Orphan Query Protein
  • 05:11Conclusion

Summary

Tadução automática

Here, we present a protocol to screen extracellular protein microarrays for identification of novel receptor-ligand interactions in high throughput. We also describe a method to enhance detection of transient protein-protein interactions by using protein-microbead complexes.

Vídeos Relacionados

Read Article