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Biologia do Desenvolvimento
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Visualization of Tangential Cell Migration in the Developing Chick Optic Tectum
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Visualization of Tangential Cell Migration in the Developing Chick Optic Tectum

Visualization of Tangential Cell Migration in the Developing Chick Optic Tectum

DOI:
10.3791/58506-v

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08:28 min

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October 24, 2018

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, ,
1Department of Neuroanatomy and Embryology, School of Medicine,Fukushima Medical University

Capítulos

  • 00:04Título
  • 00:25Electroporation In Ovo
  • 03:22Flat-Mount Culture on the Cell Insert
  • 05:39Time-Lapse Imaging
  • 06:50Results: Visualization of Tangential Cell Migration
  • 08:07Conclusion

Summary

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We describe the methods for fluorescent labeling of tangentially migrating cells by electroporation, and for time-lapse imaging of the labeled cell movement in a flat-mount culture in order to visualize migrating cell behavior in the developing chick optic tectum.

Tags

Chick Optic TectumCell MigrationBiologia do DesenvolvimentoAlkaline LysisPlasmid DNAChicken EggsEmbryo ElectroporationOptic Tectum InjectionMicropipettePBSForceps ElectrodesPulse Generator

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