Journal
/
Biologia do Desenvolvimento
/
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

DOI:
10.3791/58512-v

•

05:48 min

•

December 02, 2018

•

, , , , , ,
1State Key Laboratory of Reproductive Medicine,Nanjing Medical University, 2Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine,University of Science and Technology of China

Capítulos

  • 00:04Título
  • 00:25Inulin-FITC Administration
  • 02:20Tissue Harvest and Imaging
  • 04:00Results: Representative Blood-Testis Barrier (BTB) Integrity Assessment
  • 04:50Conclusion

Summary

Tadução automática

Tadução automática

Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.

Tags

Blood-testis BarrierIn Vivo MethodMouseCadmium ChlorideTestisInulin-FITCMicroinjectionCryosectioningParaformaldehydeOptimal Cutting Temperature Compound

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/58512?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/pt/58512?article_type=v

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
check_url/pt/58512?article_type=v

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
check_url/pt/58512?article_type=v

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
check_url/pt/58512?article_type=v

An Efficient Method to Obtain Dedifferentiated Fat Cells
check_url/pt/58512?article_type=v

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition
check_url/pt/58512?article_type=v

An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks
check_url/pt/58512?article_type=v

Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo
check_url/pt/58512?article_type=v

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues
check_url/pt/58512?article_type=v

A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome

Read Article