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用于玻璃化和变暖步骤中囊胚行为客观评估的形态学协议

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps

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用于玻璃化和变暖步骤中囊胚行为客观评估的形态学协议

DOI:

10.3791/58540-v

•

08:28 min

•

February 28, 2019

•

Marjan Taborin, Borut Kovačič

¹Department of Reproductive Medicine and Gynecological Endocrinology,University Medical Centre Maribor

Capítulos

00:04Título
00:41Blastocyst Recording During Equilibration, Cross-Sectional Area Measurement, Data Editing and Creation of a Line Diagram
03:27Blastocyst Time-Lapse Re-Expansion Recording, Measurements, Data Editing and Creation of Line Diagram
06:09Results: Representative Blastocoel Contraction and Re-Expansion Analyses
07:49Conclusion

Summary

Tadução automática

在这里, 我们提出了一个延时形态测量方案, 以跟踪在预防干预和升温后恢复过程中囊胚收缩和再膨胀的强度。该方案可在配备延时显微镜的体外受精实验室中应用, 并建议在开发最佳囊胚玻璃化方法时使用。

Tags

Keywords: Blastocyst Morphometric Vitrification Warming Shrinkage Re-expansion Time-lapse Microscopy Zona Pellucida Trophectoderm Inner Cell Mass Equilibration Medium Laser Pulse Microdroplet Cross-sectional Area In Vitro Fertilization

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