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Imunologia e Infecção

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A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

DOI:

10.3791/58574-v

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14:54 min

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January 27, 2019

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Byron Brook*¹, Nelly Amenyogbe*¹, Rym Ben-Othman, Bing Cai, Danny Harbeson, Freddy Francis, Aaron C. Liu, Natallia Varankovich, James Wynn⁴, Tobias R. Kollmann²

¹Department of Experimental Medicine,University of British Columbia, ²Department of Pediatrics, Division of Infectious Diseases,University of British Columbia, ³Department of Pediatrics, College of Medicine,University of Florida, ⁴Department of Pathology, Immunology, and Laboratory Medicine,University of Florida

Capítulos

00:00Título
00:33Preparation of Frozen Cecal Slurry Stock from Adult Mouse Donors
04:42Challenging Newborn Mice with a Weight-adjusted Dose of Cecal Slurry
08:00Monitoring Mice and Determining Humane Endpoint
10:34Representative Results
14:28Conclusion

Summary

Tadução automática

This protocol provides the necessary steps to establish and evaluate neonatal sepsis in 7-day-old mice.

Tags

Neonatal Polymicrobial Sepsis Cecal Slurry Model Standardized Procedure Data-driven Approach Moribund Pups Frozen Cecal Slurry Stock Cecal Contents Dissection Sterile Tools Biological Safety Cabinet

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