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Immunology and Infection

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JoVE Journal Immunology and Infection
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
 

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Article DOI: 10.3791/58595-v • 08:13 min • January 20th, 2019
January 20th, 2019 •
1, 2, 1, 1, 1, 1, 1, 1, 1
1Inovio Pharmaceuticals, Inc, 2Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute

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Resumo
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The development of the interferon-gamma ELISpot assay for guinea pig PBMCs allows the characterization of T-cell responses in this highly relevant model for studying infectious diseases. We have applied the assay to measure T cell responses associated with intradermal delivery of DNA vaccines.

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Keywords: ELISpot Assay T Cell Responses Guinea Pig Model Vaccination Immunotherapeutics Humoral Immunity Peripheral Blood Kinetics Capture Anti-guinea Pig Interferon Gamma Antibody VE4 Density Gradient Medium PBMCs
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