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Estimation of Nephron Number in Whole Kidney using the Acid Maceration Method
 

Estimation of Nephron Number in Whole Kidney using the Acid Maceration Method

Article DOI: 10.3791/58599-v • 08:15 min • May 22nd, 2019
May 22nd, 2019 •
1, 2, 1, 1, 1,3, 1,4
1Department of Pharmacology and Toxicology, The University of Mississippi Medical Center, 2Department of Medicine, Division of Nephrology, Rush University Medical Center, 3Department of Medicine, Division of Nephrology, The University of Mississippi Medical Center, 4Department of Neurology, The University of Mississippi Medical Center

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Resumo
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Estimates of whole kidney nephron number are important clinically and experimentally, as there is an inverse association between nephron number and an enhanced risk of renal and cardiovascular disease.Herein, the use of the acid maceration method, which provides fast and reliable estimates of whole kidney nephron number, is demonstrated.

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Keywords: Nephron Number Acid Maceration Method Kidney Renal Function Nephron Development Gene Influence Renal Pathology Magnetic Resonance Imaging Experimental Technique Dissection Kidney Weight Tissue Isolation Tissue Processing Hydrochloric Acid
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