Journal
/
Neurociência
/
Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

DOI:
10.3791/58613-v

•

08:58 min

•

June 19, 2019

•

, ,
1Department of Molecular Neuroscience and Integrative Physiology, Faculty of Medicine,Kanazawa University, 2International Institute for Integrative Sleep Medicine (WPI-IIIS),University of Tsukuba, 3Faculty of Medicine,University of Tsukuba

Capítulos

  • 00:04Título
  • 00:38Animal Preparation
  • 01:33Virus Injection
  • 02:47Electroencephalogram and Electromyogram (EEG/EMG) Electrode and Optical Fiber Implantation
  • 05:20EEG/EMG Monitoring
  • 07:19Results: Representative Effect of GABABNST Stimulation on State Transition in NREM and REM Sleep
  • 08:09Conclusion

Summary

Tadução automática

Tadução automática

Here, we describe methods of optogenetic manipulation of particular types of neurons during monitoring of sleep/wakefulness states in mice, presenting our recent work on the bed nucleus of the stria terminalis as an example.

Tags

Optogenetic ManipulationNeural CircuitsSleep/wakefulnessMiceNeuro CircuitSleep Wakefulness RegulationSurface EEGAnesthetized MouseStereotactic ApparatusAdeno-associated Virus VectorMicroinjectionBed Nucleus Of The Stria Terminalis (BNST)Electroencephalogram (EEG)Electromyogram (EMG)

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/58613?article_type=v

Extinction Training During the Reconsolidation Window Prevents Recovery of Fear
check_url/pt/58613?article_type=v

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex
check_url/pt/58613?article_type=v

Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits
check_url/pt/58613?article_type=v

In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons
check_url/pt/58613?article_type=v

Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion
check_url/pt/58613?article_type=v

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices
check_url/pt/58613?article_type=v

Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice
check_url/pt/58613?article_type=v

Disruption of Frontal Lobe Neural Synchrony During Cognitive Control by Alcohol Intoxication
check_url/pt/58613?article_type=v

The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice
check_url/pt/58613?article_type=v

Measuring Neural Mechanisms Underlying Sleep-Dependent Memory Consolidation During Naps in Early Childhood

Read Article