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Imunologia e Infecção
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Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

DOI:
10.3791/58621-v

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08:07 min

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January 07, 2019

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, ,
1Division of Gastroenterology and Hepatology, Department of Medicine,University of Colorado Anschutz Medical Campus, School of Medicine, 2Department of Immunology and Microbiology,University of Colorado Anschutz Medical Campus

Capítulos

  • 00:04Título
  • 00:52Liver Single-cell Suspension Preparation
  • 03:36Flow Cytometric Analysis
  • 05:49Results: Representative Murine Liver Lymphatic Endothelial Cell (LEC) Characterization
  • 07:22Conclusion

Summary

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The goal of this protocol is to identify lymphatic endothelial cell populations within the liver using described markers. We utilize collagenase IV and DNase and a gentle mincing of tissue, combined with flow cytometry, to identify a distinct population of lymphatic endothelial cells.

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LiverLymphatic Endothelial CellsFlow CytometrySingle-cell SuspensionLiver Biology

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