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小鼠肝脏消化对淋巴内皮细胞的流式细胞仪分析
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells
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小鼠肝脏消化对淋巴内皮细胞的流式细胞仪分析

DOI:
10.3791/58621-v

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08:07 min

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January 07, 2019

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, ,
1Division of Gastroenterology and Hepatology, Department of Medicine,University of Colorado Anschutz Medical Campus, School of Medicine, 2Department of Immunology and Microbiology,University of Colorado Anschutz Medical Campus

Capítulos

  • 00:04Título
  • 00:52Liver Single-cell Suspension Preparation
  • 03:36Flow Cytometric Analysis
  • 05:49Results: Representative Murine Liver Lymphatic Endothelial Cell (LEC) Characterization
  • 07:22Conclusion

Summary

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该方案的目标是使用所述标记识别肝脏内的淋巴管内皮细胞群。我们利用胶原酶 iv 和 dnase 和组织的温和切碎, 结合流式细胞仪, 以确定一个独特的淋巴内皮细胞的群体。

Tags

Keywords: LiverLymphatic Endothelial CellsFlow CytometrySingle-cell SuspensionLiver Biology

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