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用病毒载体接种后, mhc i 四分体染色同时定量抗载体和抗转基因特异性 cd8+ t 细胞
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
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用病毒载体接种后, mhc i 四分体染色同时定量抗载体和抗转基因特异性 cd8+ t 细胞

DOI:
10.3791/58680-v

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08:10 min

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November 28, 2018

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, , ,
1Division of Virology, Department of Hygiene, Microbiology, Social Medicine,Medical University of Innsbruck

Capítulos

  • 00:04Título
  • 00:49Sample Collection
  • 01:21Staining Set-up
  • 01:59Tetramer Staining and Preparation of Antibodies
  • 03:17Staining of Samples
  • 04:24Lysis of Erythrocytes
  • 05:09Flow Cytometric Measurement and Analysis
  • 06:53Results: Quantification of Antigen-specific CD8+ T Cells in Mouse Blood of Lymphoid Organs
  • 07:36Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们提出了一个协议的体外定性检测抗原特异性 cd8+ t 细胞。从器官或少量血液中进行单细胞悬浮液分析是可能的。广泛的研究需要分析细胞毒性 t 细胞的反应 (疫苗接种和癌症免疫治疗研究)。

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