Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Visualization of 3D White Adipose Tissue…
JoVE Journal
Biology

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Biology
Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining
 

Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining

Article DOI: 10.3791/58683-v • 06:20 min • November 17th, 2018
November 17th, 2018 •
*1, *1, 1,2, 1, 3, 4, 1,2,5
1Translational Medicine Program, The Hospital for Sick Children, 2Department of Laboratory Medicine and Pathobiology, University of Toronto, 3Neurosciences & Mental Health Program, The Hospital for Sick Children, 4Department of Biochemistry and Molecular Biology, Smart-Aging Convergence Research Center, College of Medicine, Yeungnam University, 5Banting and Best Diabetes Centre, University of Toronto
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

The focus of the present study is to demonstrate the whole-mount immunostaining and visualization technique as an ideal method for 3D imaging of adipose tissue architecture and cellular component.

Tags

Keywords: 3D Visualization Whole-mount Staining Adipose Tissue Tissue Structure Metabolic Conditions Fasting Obesity Tissue Processing Immunohistochemistry Tissue Dissection Fixation Antibody Staining Blocking Primary Antibodies Secondary Antibodies Neutral Lipid Staining Tissue Washing
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter