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Imunologia e Infecção
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Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

DOI:
10.3791/58688-v

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06:54 min

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June 15, 2019

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, , , , , , , ,
1Department of Life Sciences,University of Trieste, 2Institute for Maternal and Child Health,IRCCS (Istituto di Ricovero e Cura a Carattere Scientifico) Burlo Garofolo, 3Department of Medical, Surgical and Health Science,University of Trieste

Capítulos

  • 00:00Título
  • 00:59HA and C1q Coating Procedure
  • 02:45Cells Labelling with Fast Dil
  • 04:04Cells Adhesion on HA/C1q Matrices
  • 05:32Resultados
  • 06:02Conclusion

Summary

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The complement component C1q is a pro-inflammatory molecule highly expressed in the tissue microenvironment that can interact with the extracellular matrix. Here, we describe a method to test how C1q bound to hyaluronic acid impacts cell adhesion.

Tags

C1qHyaluronic AcidCell AdhesionExtracellular MatrixInnate Immune SystemTumor MicroenvironmentPlacenta MicroenvironmentCell DivisionBinary CellsCoating ProcedureEnzyme-linked ImmunoassayFast DiICell Labeling

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