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JoVE Journal Immunology and Infection
Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
 

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Article DOI: 10.3791/58688-v • 06:54 min • June 15th, 2019
June 15th, 2019 •
*1, *2, 1, 1, 2, 3, 3, 2,3, 1
1Department of Life Sciences, University of Trieste, 2Institute for Maternal and Child Health, IRCCS (Istituto di Ricovero e Cura a Carattere Scientifico) Burlo Garofolo, 3Department of Medical, Surgical and Health Science, University of Trieste
* These authors contributed equally

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The complement component C1q is a pro-inflammatory molecule highly expressed in the tissue microenvironment that can interact with the extracellular matrix. Here, we describe a method to test how C1q bound to hyaluronic acid impacts cell adhesion.

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Keywords: C1q Hyaluronic Acid Cell Adhesion Extracellular Matrix Innate Immune System Tumor Microenvironment Placenta Microenvironment Cell Division Binary Cells Coating Procedure Enzyme-linked Immunoassay Fast DiI Cell Labeling
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