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1Department of Cell, Developmental, & Cancer Biology,Oregon Health and Science University, 2Department of Molecular Microbiology & Immunology,Oregon Health and Science University, 3Department of Dermatology,Oregon Health and Science University, 4Knight Cancer Institute,Oregon Health and Science University
Capítulos
- 00:04Título
- 00:46Mouse Pinna Inflammation Induction, Vaccinia Infection, Photoconversion, and Tissue Processing
- 02:52Intradermal Melanoma Tumor Photoconversion and Tissue Processing
- 04:00Flow Cytometry Preparation & Analysis
- 05:18Results: Representative Photoconversion Efficiency in Skin and the Tumor Microenvironments
- 07:55Conclusion
Ici, nous montrons les méthodes in vivo la quantification d’évacuation de leucocytes de naïve, enflammée et peau murine maligne. Nous effectuons une comparaison entre deux modèles : transdermique FITC application et in situ photoconversion. En outre, nous démontrent l’utilité de photoconversion pour le suivi des sorties de leucocytes de tumeurs cutanées.
