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An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis

An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis

DOI:
10.3791/58713-v

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06:35 min

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February 08, 2019

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, , , ,
1Department of Medicine,Penn State University, 2Department of Pathology,Penn State University, 3Penn State College of Medicine, 4Department of Oncology,Wayne State University School of Medicine

Capítulos

  • 00:04Título
  • 00:46Temporal Artery Biopsy Preparation and Processing
  • 01:33Dewaxing, Antigen Retrieval, and Staining
  • 02:24Immunohistochemical Preparation
  • 04:07Histological and Immunohistochemical (IHC) Analyses
  • 04:45Results: Representative Temporal Artery Imaging
  • 06:05Conclusion

Summary

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The protocol illustrates the use of histopathologic examination and immunohistochemistry to profile the folate receptor beta macrophage and its relationship with the total immune cell infiltrate in temporal artery biopsies in giant cell arteritis.

Tags

Folate Receptor BetaImmunohistochemistryGiant Cell ArteritisTemporal ArteryVascular MicroenvironmentMacrophagesAntigen RetrievalImmunohistopathology

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