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Capítulos
- 00:04Título
- 00:31Growing Cells on Acoustically-Transparent Polyester Film
- 02:34LIPUS Implementation and Oblique Acoustic Excitation
- 03:29Driving the Transducer
- 04:24Beam Alignment and Determination of Ultrasound Pulse Pressure and Intensity
- 06:32Calcium-Sensitive/LIPUS Live-Cell Fluorescence Imaging
- 07:33Results: LIPUS-induced Calcium Signals in Human Glioblastoma Cells A-172
- 08:20Conclusion
Low-Intensity Pulsed Ultrasound Stimulation (LIPUS) is a modality for non-invasive mechanical stimulation of endogenous or engineered cells with high spatial and temporal resolution. This article describes how to implement LIPUS to an epi-fluorescence microscope and how to minimize acoustic impedance mismatch along the ultrasound path to prevent unwanted mechanical artefacts.
