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减法基因组学的新序列发现

JoVE Journal
Genética

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JoVE Journal Genética

Novel Sequence Discovery by Subtractive Genomics

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减法基因组学的新序列发现

DOI:

10.3791/58877-v

•

09:40 min

•

January 25, 2019

•

Kathryn C. Asalone, Megan M. Nelson, John R. Bracht

¹Biology Department,American University

Capítulos

00:04Título
01:08De novo Assemble Starting Sequence
02:03BLAST the Assembly Against the Reference Sequence
04:44Design Primers for the Sequence that Remains
06:25qPCR Validation of the Remaining Sequence
07:51Results: Example Data of qPCR Results Including Negative and Positive Outcomes
08:53Conclusion

Summary

Tadução automática

该协议的目的是使用计算和工作台研究的组合来寻找无法轻易从共同净化序列中分离的新序列, 这可能只是部分已知的序列。

Tags

Novel Sequence Discovery Subtractive Genomics Genomic Sequences Inexpensive Flexible Bacterial Vaccine Targets Virus Identification Unknown Separation Reference Sequence Trimmomatic Pear Reptile Trinity Blast Transdeckor Tblastn

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