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Interfacing Microfluidics with Microelectrode Arrays for Studying Neuronal Communication and Axonal Signal Propagation
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JoVE Journal Bioengenharia
Interfacing Microfluidics with Microelectrode Arrays for Studying Neuronal Communication and Axonal Signal Propagation

Interfacing Microfluidics with Microelectrode Arrays for Studying Neuronal Communication and Axonal Signal Propagation

DOI:
10.3791/58878-v

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11:27 min

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December 08, 2018

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, ,
1i3S – Instituto de Investigação e Inovação em Saúde,Universidade do Porto, 2INEB – Instituto de Engenharia Biomédica,Universidade do Porto, 3ICBAS – Instituto de Ciências Biomédicas Abel Salazar,Universidade do Porto

Capítulos

  • 00:00Título
  • 01:37Microfluidic and MEA Devices Preparation
  • 03:55Prefrontal Cortex Dissection
  • 05:01Cortical Neurons Dissociation and Culture
  • 08:03Recording and Data Analysis
  • 09:58Representative Results
  • 11:00Conclusion

Summary

Tadução automática

Tadução automática

This protocol aims to demonstrate how to combine in vitro microelectrode arrays with microfluidic devices for studying action potential transmission in neuronal cultures. Data analysis, namely the detection and characterization of propagating action potentials, is performed using a new advanced, yet user-friendly and freely available, computational tool.

Tags

MicrofluidicsMicroelectrode ArraysNeuronal CommunicationAxonal Signal PropagationNeural CircuitsNeural CodingNeural DecodingSensory NeuronsCell CultureData AnalysisComputational ToolsPEI CoatingMEA Preparation

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