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Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets
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Neurociência
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JoVE Journal Neurociência
Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

DOI:
10.3791/58879-v

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10:16 min

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March 12, 2019

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, , ,
1Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences,University of Copenhagen, 2Neurobiology Research Unit and CIMBI,Copenhagen University Hospital, 3Department of Clinical Physiology, Nuclear Medicine & PET,Copenhagen University Hospital

Capítulos

  • 00:04Título
  • 00:46Tissue Preparation by Cryosectioning
  • 02:13In vitro Autoradiography
  • 04:44Exposure to Phosphor Imaging Plates and Scanning
  • 06:04Densitometric Analysis of Digital Image
  • 07:34Results: Visualization and Characterization of Pharmacological Targets Using Radiography
  • 09:34Conclusion

Summary

Tadução automática

Tadução automática

The method of autoradiography is routinely used to study binding of radioligands to tissue sections for determination of qualitative or quantitative pharmacology.

Tags

AutoradiographyProtein BindingPharmacological TargetsCryostat SectioningRadioligand LabelingNon-specific BindingSpatial ResolutionDigital ImagingPharmacological Analysis

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