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Journal / Biochemistry / A Fluorogenic Peptide Cleavage Assay to Screen…
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Biochemistry

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JoVE Journal Biochemistry
A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity
 

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Article DOI: 10.3791/58892-v • 07:53 min • January 9th, 2019
January 9th, 2019 •
*1,2, *1,3, 1,4, 1, 1
1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 3Virologie et Immunologie Moléculaires, Domaine de Vilvert, INRA, 4Department of Cell Biology and Molecular Genetics, University of Maryland
* These authors contributed equally

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We present a fluorogenic peptide cleavage assay that allows a rapid screening of the proteolytic activity of proteases on peptides representing the cleavage site of viral fusion peptides. This method can also be used on any other amino acid motif within a protein sequence to test for the protease activity.

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Keywords: Fluorogenic Peptide Cleavage Assay Proteolytic Activity Coronavirus Spike Protein Activation Proteolytic Modification Protease Characterization Viral Fusion Peptide Design FRET Pair Fluorescence Plate Reader Protease Activity Assay
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