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Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
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Capítulos
- 00:04Título
- 00:38Fixation of Tumor NS
- 01:21Paraffin Embedding of Tumor NS
- 01:51Sectioning of Paraffin-Embedded NS
- 03:23Immunohistochemistry (IHC) of Paraffin-Embedded NS
- 05:31Results: NS Generated, Analyzed via Bioluminescence, and Stained for Glioma Biomarkers
- 06:10Conclusion
Neurospheres grown as 3D cultures constitute a powerful tool to study glioma biology. Here we present a protocol to perform immunohistochemistry while maintaining the 3D structure of glioma neurospheres through paraffin embedding. This method enables the characterization of glioma neurosphere properties such as stemness and neural differentiation.
