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石蜡包埋三维胶质瘤神经球培养的免疫组织化学评价胶质瘤生物标志物

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Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Please note that all translations are automatically generated. Click here for the English version.

石蜡包埋三维胶质瘤神经球培养的免疫组织化学评价胶质瘤生物标志物

DOI:

10.3791/58931-v

•

06:32 min

•

January 09, 2019

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Felipe J. Núñez², Flor M. Mendez, Maria B. Garcia-Fabiani², Joaquín Pardo³, Marta Edwards², Pedro R. Lowenstein², Maria G. Castro²

¹Department of Neurosurgery,University of Michigan Medical School, ²Department of Cell & Developmental Biology,University of Michigan, ³INIBIOLP, Histology B-Pathology B, School of Medicine,UNLP

Capítulos

00:04Título
00:38Fixation of Tumor NS
01:21Paraffin Embedding of Tumor NS
01:51Sectioning of Paraffin-Embedded NS
03:23Immunohistochemistry (IHC) of Paraffin-Embedded NS
05:31Results: NS Generated, Analyzed via Bioluminescence, and Stained for Glioma Biomarkers
06:10Conclusion

Summary

Tadução automática

作为3d 培养体生长的神经球是研究胶质瘤生物学的有力工具。在这里, 我们提出了一个执行免疫组织化学的协议, 同时保持三维结构的胶质瘤神经球通过石蜡嵌入。这种方法可以描述胶质瘤神经球的性质, 如干细胞和神经分化。

Tags

Keywords: 3D Glioma Neurosphere Cultures Immunohistochemistry Paraffin-embedded Biomarkers Microtome Sectioning

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