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在 Vivo 钙成像中，在 C. elegans身体壁肌肉

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

In Vivo Calcium Imaging in C. elegans Body Wall Muscles

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在 Vivo 钙成像中，在 C. elegans身体壁肌肉

DOI:

10.3791/59175-v

•

08:03 min

•

October 20, 2019

•

Ashley A. Martin², Simon Alford, Janet E. Richmond

¹Department of Biological Sciences,University of Illinois at Chicago, ²Department of Integrative Biology and Physiology,University of Minnesota, ³Department of Anatomy and Cell Biology,University of Illinois at Chicago

Capítulos

00:04Título
01:10Microscope Setup
02:57C. elegans Sample Preparation & Data Acquisition
05:16Results: Baseline Calcium Levels & Evoked Calcium Transients in C. elegans Body Wall Muscles
07:03Conclusion

Summary

Tadução automática

这种方法提供了一种方法，将光遗传学和基因编码的钙传感器相结合，以成像基线细胞钙水平，以及模型生物体C.elegans身体壁肌肉中诱发的钙瞬变的变化。

Tags

Keywords: Calcium Imaging C. Elegans Body Wall Muscles Calcium Homeostasis Calcium Handling Neurobiology Biologia do Desenvolvimento Cell Biology Nematodes Microscopy Fluorescent Imaging CMOS Camera Micromanager Software ImageJ Channel Rhodopsin RCaMP LED Illumination TTL Signals Power Supplies

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