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用细胞遗传学技术测定芽孢杆菌的受精率

JoVE Journal
Genética

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JoVE Journal Genética

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

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用细胞遗传学技术测定芽孢杆菌的受精率

DOI:

10.3791/59213-v

•

05:24 min

•

April 01, 2019

•

Elizabeth C. Bondy, Martha S. Hunter

¹Graduate Interdisciplinary Program in Entomology and Insect Science,University of Arizona, ²Department of Entomology,University of Arizona

Capítulos

00:04Título
00:31Initial Setup and Dechorionation
02:11Fixation, Staining, Washing, and Mounting
03:49Results: Influence of Rickettsia sp. nr. bellii Endosymbiont on Primary Sex Ratio of B. tabaci
04:51Conclusion

Summary

Tadução automática

我们提出了一个简单的细胞遗传学技术, 利用 4 ', 6-二胺-2-苯基苯二酚 (dapi) 来确定施肥率和主要性别比例的单倍体入侵害虫杆菌。

Tags

Keywords: Bemisia Tabaci Whitefly Cytogenetic Technique Egg Fertilization Rate Haplodiploid Sex Ratio Oviposition Dechorionation DAPI Staining Microscopy

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