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Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue
DOI:

12:05 min

February 13, 2019

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Capítulos

  • 00:04Título
  • 00:53Adipose Tissue Collection and Antibody Incubation
  • 05:17Image Acquisition and Analysis of Blood Vessel and Nerve Fiber Networks
  • 09:37Results: Whole Mount Co-immunofluorescence Staining of Blood Vessels and Nerve Fibers in Adipose Tissues
  • 11:17Conclusion

Summary

Tadução automática

New blood vessel formation and sympathetic innervation play pivotal roles in adipose tissue remodeling. However, there remain technical issues in visualizing and quantitatively measuring adipose tissue. Here we present a protocol to successfully label and quantitatively compare the densities of blood vessels and nerve fibers in different adipose tissues.

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