Journal
/
Bioengenharia
/
Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
JoVE Journal
Bioengenharia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioengenharia
Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

DOI:
10.3791/59285-v

•

04:59 min

•

April 12, 2019

•

,
1Department of Biology,Harper College

Capítulos

  • 00:04Título
  • 00:30Medium Preparation
  • 01:28S. aureus Biofilm Formation in 96 Well Tissue Culture Plate
  • 02:48Measurement of Alkaline Phosphatase (ALP) Activity in S. aureus Biofilm
  • 03:45Results: Alkaline Phosphatase Activity
  • 04:10Conclusion

Summary

Tadução automática

Tadução automática

In this manuscript, we established a high throughput method to quantify alkaline phosphatase activity in S. aureus biofilm culture from 96-well tissue culture plate

Tags

Alkaline Phosphatase ActivityS. AureusBiofilmColorimetric AnalysisHigh-throughputTSBTSAGlucose96-well PlatePNPPSodium Hydroxide

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/59285?article_type=v

Quantification of Global Diastolic Function by Kinematic Modeling-based Analysis of Transmitral Flow via the Parametrized Diastolic Filling Formalism
check_url/pt/59285?article_type=v

Protocol for Biofilm Streamer Formation in a Microfluidic Device with Micro-pillars
check_url/pt/59285?article_type=v

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants
check_url/pt/59285?article_type=v

A Zebrafish Embryo Model for In Vivo Visualization and Intravital Analysis of Biomaterial-associated Staphylococcus aureus Infection
check_url/pt/59285?article_type=v

High-resolution Patterned Biofilm Deposition Using pDawn-Ag43
check_url/pt/59285?article_type=v

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels
check_url/pt/59285?article_type=v

Home-Based Monitor for Gait and Activity Analysis
check_url/pt/59285?article_type=v

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images
check_url/pt/59285?article_type=v

Electric and Magnetic Field Devices for Stimulation of Biological Tissues
check_url/pt/59285?article_type=v

Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting

Read Article