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利用成熟的细胞隔离试剂盒快速分离脂滴器

JoVE Journal
Biologia

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JoVE Journal Biologia

Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit

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利用成熟的细胞隔离试剂盒快速分离脂滴器

DOI:

10.3791/59290-v

•

08:43 min

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April 19, 2019

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Jascha Brettschneider*¹, Jason M. Correnti*¹, Chelsea Lin, Bianca Williams, Amanke Oranu, Amy Kuriakose, Dru McIver-Jenkins, Abigail Haba, Isabelle Kaneza, Sookyoung Jeon, Eleonora Scorletti, Rotonya M. Carr

¹Division of Gastroenterology,Perelman School of Medicine, University of Pennsylvania

Capítulos

00:04Título
00:54Tissue Preparation
03:10Lipid Droplet (LD) Isolation
05:18LD Washing
06:38Results: Analysis of LDs after Isolation
08:19Conclusion

Summary

Tadução automática

该方案建立了一种新的方法, 从小鼠肝脏脂滴分离和纯化, 使用一个完善的内质网隔离试剂盒。

Tags

Keywords: Lipid Droplet Organelle Isolation Liver Mouse ER Lysosome Homogenization Centrifugation IE Buffer Rapid Isolation

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