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Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels
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Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels

Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels

DOI:
10.3791/59304-v

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11:01 min

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July 24, 2019

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, , , ,
1Department of Chemical and Biomolecular Engineering,Vanderbilt University, 2Department of Radiation Oncology,Stanford University, 3Depattment of Biomedical Engineering,Vanderbilt University, 4Department of Radiation Oncology,Vanderbilt University Medical Center

Capítulos

  • 00:04Título
  • 00:45Preparation and Ex Vivo Irradiation of MFPs
  • 01:13Decellularization
  • 06:00Milling
  • 06:51Hydrogel Formation
  • 07:44Encapsulating Cells in Hydrogels
  • 08:33Results: Studying Normal Tissue Radiation Effects
  • 09:44Conclusion

Summary

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Tadução automática

This protocol presents a method for decellularization and subsequent hydrogel formation of murine mammary fat pads following ex vivo irradiation.

Tags

Extracellular MatrixHydrogelsRadiation EffectsDecellularizationMammary Fat PadBreast CancerRecurrence MechanismsTrypsin-EDTAT-octylphenoxypolyethoxyethanolDeoxycholic Acid

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