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使用卡内哈布迪炎酶研究有毒物质的跨代和多代效应

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Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants

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使用卡内哈布迪炎酶研究有毒物质的跨代和多代效应

DOI:

10.3791/59367-v

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08:58 min

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July 29, 2019

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Zhuo Li², Fangting Ai, Jing Zhang, Zhenyang Yu², Daqiang Yin²

¹College of Environmental Sciences and Engineering,Tongji University, ²Shanghai Institute of Pollution Control and Ecological Security, ³Jiaxing Tongji Institute of Environment, ⁴College of Ecological Technique and Engineering,Shanghai Institute of Technology

Capítulos

00:04Título
01:16Culture E. coli OP50 and C. elegans
02:00Prepare Synchronized Eggs and L3 Larvae of C. elegans
04:12Use C. elegans for Trans-generational Effect Study
05:58Use C. elegans for Multi-generational Exposure (MGE) Effect Study
07:04Results: Trans-generational (TG), Multi-generational Exposure (MGE) and Multi-generational Residual (MGR) Effect Studies
08:08Conclusion

Summary

Tadução automática

持久性化学品的跨代和多代影响对于判断其对环境和人类健康的长期影响至关重要。我们提供了研究跨代和多代效应使用自由生活线虫卡埃诺哈布迪炎埃莱甘斯的新方法。

Tags

Keywords: Caenorhabditis Elegans Trans-generational Effects Multi-generational Effects Toxicants Nematode Hermaphrodites Pharmaceutical Synthesis Environmental Management Aquatic Toxicity Daphnia Magna Drosphila Melanogaster E. Coli LB Medium NGM Agars Gravid Nematodes Age-synchronization Sodium Hypochlorite Solution

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