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不同植物种的射击顶端共聚焦活图

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species

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不同植物种的射击顶端共聚焦活图

DOI:

10.3791/59369-v

•

06:46 min

•

March 29, 2019

•

Yuan Geng², Yun Zhou²

¹Department of Botany and Plant Pathology,Purdue University, ²Purdue Center for Plant Biology,Purdue University

Capítulos

00:04Título
00:36Plant Growth and Dissection of the Shoot Apex
01:43Staining and Image Collection
03:38Image Processing
04:43Results: Live Imaging and Analyzing of the Inflorescence Shoot Apical Meristem of Arabidopsis and the Vegetative Shoot Apical Meristem of Tomato and Soybean
06:19Conclusion

Summary

Tadução automática

该协议介绍了如何利用激光扫描共聚焦显微镜对不同植物种类的芽顶端分生组织进行成像和分析。

Tags

Keywords: Confocal Live Imaging Shoot Apical Meristem Plant Species Arabidopsis Tomato Soybean Propidium Iodide Staining Dissection Sample Preparation Confocal Microscopy

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