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功能金报告系统测量特定分枝杆菌误调速率
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems
DOI:

06:18 min

April 26, 2019

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Capítulos

  • 00:04Título
  • 00:52Renilla-firefly Dual-luciferase Reporter
  • 03:02Nluc/GFP Reporter
  • 05:08Results: Measuring Rates of Translational Error Using Gain-of-function Reporter Systems
  • 05:50Conclusion

Summary

Tadução automática

在本文中, 我们提出了两种互补的方法来测量特定的率的平移误差和误译的模型分枝杆菌, 分枝杆菌 smatis, 使用功能增益报告系统。这些方法可用于测量低吞吐量或更高吞吐量设置中的相对错误率的准确错误率。

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