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Imunologia e Infecção
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A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia

A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia

DOI:
10.3791/59566-v

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00:13 min

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September 21, 2019

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, , ,
1Department of Microbiology and Immunology,Montana State University, 2University Communications,Montana State University

Capítulos

  • 00:01Título
  • 02:06Intratracheal Instillation
  • 04:46Excision of Infected Lungs
  • 07:16Pathogen Recovery and Analysis
  • 10:03Resultados
  • 12:06Conclusion

Summary

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Here, we present methods to improve secondary bacterial pneumonia studies by providing a non-invasive route of instillation into the lower respiratory tract followed by pathogen recovery and transcript analysis. These procedures are reproducible and can be performed without specialized equipment such as cannulas, guide wires, or fiber optic cables.

Tags

Secondary Bacterial PneumoniaMurine ModelPathogen DeliveryPathogen RecoveryTracheal InoculationBacterial CFU EnumerationRNA PurificationVirulence Gene AnalysisMinimally Invasive Technique

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