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Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
JoVE Journal
Biologia
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JoVE Journal Biologia
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

DOI:
10.3791/59577-v

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05:58 min

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July 17, 2019

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, , , ,
1Stowers Institute for Medical Research, 2Department of Molecular and Integrative Physiology,The University of Kansas School of Medicine

Capítulos

  • 00:04Título
  • 00:56Saccharomyces cerevisiae Preparation
  • 02:04Photoconversion and Distributed Amphifluoric Fluorescence Resonance Energy Transfer (DAmFRET) Data Collection
  • 02:51Data Analysis
  • 03:32Results: Representative In Vivo Protein Self-Assembly Detection and Characterization
  • 05:26Conclusion

Summary

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This article describes a FRET-based flow cytometry protocol to quantify protein self-assembly in both S. cerevisiae and HEK293T cells.

Tags

Keywords: Protein Self-assemblyFlow CytometryDAmFRETFRETAcceptor SignalYeast CultureInduction MediumPhoto ConversionImaging Flow CytometerFluorescence Positive Gate

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